A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter.

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A light-regulated DNA-binding activity interacts with a conserved region of a Lemna gibba rbcS promoter.

We have characterized a DNA-binding activity, designated light-regulated nuclear factor (LRF-1), which interacted with a specific sequence located 150 nucleotides upstream from the transcription start site of a phytochrome-regulated Lemna gibba rbcS gene (SSU5B). There was a higher level of LRF-1 activity recovered from nuclei of light-grown plants than from dark-treated plants. In light-grown ...

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A Light - Regulated DNA - Binding Activity lnteracts with a Conserved Region of a Lemna gibba rbcS Promoter Jeffrey

We have characterized a DNA-binding activity, designated light-regulated nuclear factor (LRF-l), which interacted with a specific sequence located 150 nucleotides upstream from the transcription start site of a phytochromeregulated Lemna gibba rbcS gene (SSUSS). There was a higher level of LRF-1 activity recovered from nuclei of light-grown plants than from dark-treated plants. In light-grown p...

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Green Tissue-Specific Analysis of a Cloned rbcS Promoter from Lemna gibba

Wang Y. (2014): Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibba. Czech J. Genet. Plant Breed., 50: 235–240. Many plant genetic engineering taskss require the spatial expression of genes which in turn depends upon the availability of specific promoters. The present paper analyses the green-tissue characteristics of a new L. gibba rbcS promoter driving the expression of ...

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A metal-dependent DNA-binding protein interacts with a constitutive element of a light-responsive promoter.

We have used DNase I footprinting to characterize nuclear factors that bind to the light-responsive promoter of pea rbcS-3A, one member of the gene family encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. A sequence-specific binding activity, designated 3AF1, binds to an AT-rich sequence present at the -45 region of the rbcS-3A promoter. A tetramer of the 3AF1 binding site, d...

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Post-transcriptional regulation of organ-specific expression of individual rbcS mRNAs in Lemna gibba.

Many studies of nuclear genes encoding chloroplast proteins have focused on the transcriptional regulation of their expression. The genes (rbcS) encoding the small subunit (SSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase, a major stromal protein, comprise one such group. We have examined the role played by post-transcriptional events in determining the relative levels of individual rbcS...

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ژورنال

عنوان ژورنال: The Plant Cell

سال: 1990

ISSN: 1040-4651,1532-298X

DOI: 10.1105/tpc.2.8.805